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The Relationship of Endothelial Dysfunction with the Development of Diastolic Heart Failure in Patients with Hypertension

https://doi.org/10.20996/1819-6446-2020-05-04

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Abstract

Aim. To identify genetic predictors of diastolic dysfunction and signs of endothelial dysfunction in relation to indicators of left ventricular myocardial deformity in patients with hypertension.

Material and methods. The study included 74 patients (38 women and 36 men) with hypertension 1-2 stages observed in the cardiology department of the Road clinical hospital at Chita II station. The mean age of patients was 32±9.4 years. Circulating endothelial cells (CEC) were determined by flow cytometry with BT 5000 device and antibodies to CD146+, CD45. Besides, 7AAD ion dye was used to detect living and dead endothelial cells. The number of CD146+, CD45 cells per 500000 blood cells was determined. Changes in CEC, Living/Dead CEC were studied. The data was processed using CXP Cytometer and Kaluza programs. The standard echocardiography, Doppler echocardiography and tissue Doppler echocardiography were used. Frequency distribution of alleles and genotypes: 1 mutation nitric oxide synthase 3 gene NOS3 -786 T>C; (С-786Т) in relation to indicators of left ventricular myocardial deformity was analyzed in hypertensive patients with or without diastolic dysfunction as well as in the control group. Polymerase chain reaction with detection of amplification product in real time (RCR-rt) or electrophoresis in agarose gel on genomic DNA of peripheral blood leukocytes were used for studying gene mutations.

Results. Development of diastolic dysfunction in hypertensive patients enhanced with the carrier of the genotype NOS3 -786 CC (р=0.01 2, x2=1 2.53), endothelial dysfunction was manifested by an increase in the number of circulating endotheliocytes and was associated with a decrease in global longitudinal deformation of the left ventricle.

Conclusion. Endothelial dysfunction in patients with hypertension is associated with a violation of global longitudinal deformation; and in patients with diastolic dysfunction these abnormalities are more pronounced than in patients without diastolic dysfunction.

About the Authors

T. V. Kalinkina
Chita State Medical Academy
Russian Federation

Tatiana V. Kalinkina - MD, PhD, Associate Professor, Chair of Propedeutics of Internal Diseases, Chita State Medical Academy.

Gorkogo ul. 39A, Chita, 672000.



N. V. Lareva
Chita State Medical Academy
Russian Federation

Natalia V. Lareva - MD, PhD, Professor, Head of Chair of Therapy, Chita State Medical Academy.

Gorkogo ul. 39A, Chita, 672000.



M. V. Chistyakova
Chita State Medical Academy
Russian Federation

Marina V. Chistyakova - MD, PhD, Professor, Chair of Functional and Ultrasonic Diagnostics, Chita State Medical Academy.

Gorkogo ul. 39A, Chita, 672000.



V. V. Gorbunov
Chita State Medical Academy
Russian Federation

Vladimir V. Gorbunov - MD, PhD, Professor, Chair of Propaedeutics of Internal Diseases, Chita State Medical Academy.

Gorkogo ul. 39A, Chita, 672000.



References

1. Kalinkina T.V., Lareva N.V., Chistyakova M.V. Modern methods of diagnosis of left ventricular diastolic dysfunction in patients with hypertension. Trans-Baikal Medical Bulletin. 2016;2:115-20 (In Russ.)

2. Karaseva N.V., Goncharova E.V. Indicators of myocardial diastolic function in patients with obstructive sleep apnea syndrome. Siberian Medical Journal. 2016;145(2):12-5 (In Russ.)

3. Sheter N.M., Omelyanenko M.G., Plekhanov V.G., et al. Effect of coronary stenting on left ventricular diastolic dysfunction in patients with stable angina pectoris. International Journal of Interventional Cardiology. 2011;24:130-1 (In Russ.) DOI: 10.20534/ESR-16-11.12-97-102.

4. Magrina-Mercado R.M., Ramurez-Rentena C., Almeida-Gutierrez E., et al. Evaluation of endothelial function and diastolic function in patients with primary hyperparathyroidism before and after parathyroidectomy. Cir Cir. 2019;87(2):196-204. DOI:10.24875/CIRU.18000520.

5. Koch N.V., Slepukhina A.A., Lifshits G.I. Arterial hypertension: molecular genetic and pharmacological approaches. Pharmacogenetics and Pharmacogenomics. 2015;2:4-8 (In Russ.)

6. Pakhomia N.S., Uryasjev O.M. Polymorphism of some genes-candidates of cardiovascular diseases in patients with bronchial asthma with concomitant hypertensive disease. Zemskii Vrach. 2015;4:24-9 (In Russ.)

7. Rodriguez-Portelles A., Rodriguez-Leyva D. Endothelial and left ventricular diastolic function in young adults exposed to tobacco. Can J Physiol Pharmacol. 2019;97(10):1006-11. DOI:10.1139/cjpp-2019-0187.

8. Wunderlich T, Frey N., Kahler W., et al. Influence of hyperoxia on diastolic myocardial and arterial endothelial function. Undersea Hyperb Med. 2017;44(6):521-33. DOI:10.22462/11.12.2017.3.

9. Bogdanov D.V., Samyshkina N.E. Study polymorphisms of the renin-angiotensin system in hypertrophic cardiomyopathy and arterial hypertension. Bulletin of the Ural Medical Academic Science. 2015;3:56-9 (In Russ.)

10. Zimnitskaya O.V., Petrova M.M. Features of endothelial dysfunction in essential hypertension. Med-itsinskii Alfavit. 2019;3(1):29-33 (In Russ.)

11. Alekhin M.N. Problems and prospects of echocardiographic evaluation of left ventricular diastolic function. Kardiologiia. 2017;57(1):71-5 (In Russ.)

12. Alekhin M.N. Echocardiography value in patients with arterial hypertension. Kardiologiia. 2018;58(1):90-100 (In Russ.)

13. Drapkina O.M., Gaburova A.N. Diastolic heart failure: mechanisms of development and prospects impact on them. Heart Failure. 2012;5(73):310-6 (In Russ.)

14. Recommendations for the detection of subclinical lesions of target organs, CVD, CVB and CKD in patients with hypertension. Cardiology Bulletin. 2015;1:11 (In Russ.)

15. Bedirian R., Neves M.F., Oigman W., et al. Correlation between Diastolic Function and Endothelial Function in Patients with Type 2 Diabetes and Hypertension. Open Cardiovasc Med J. 2016;10:212-20. DOI:10.2174/1874192401610010212.

16. He L., Jiang Y., Su H., et al. Vascular Endothelial Function Assessed by Postischemic Diastolic Blood Pressure Is Associated with Acclimatization and Acute Mountain Sickness. High Alt Med Biol. 2016;17(1):11-5. DOI:10.1089/ham.2015.0070.

17. Ageev F.T. Diastolic heart failure: 10 years of Dating. Heart Failure. 2010;1(57):69-76 (In Russ.)

18. Posokhov I.N. Isolated systolic arterial hypertension of young people: clinical case. Arterial Hypertension. 2015;21(5):530-4 (In Russ.)

19. Marsalskaya O.A., Nikiforov V.S. Logitudinal deformation of the left ventricle and of the left atrium in workers of railway transport with arterial hypertension. Systemic Hypertension. 2015;12(4):18-22 (In Russ.)

20. Obrezan A. G., Baranov D.Z. Myocardial strain properties in patients with chronic heart failure. Kardiologiia. 2019;59(8):88-96. (In Russ.)


For citation:


Kalinkina T.V., Lareva N.V., Chistyakova M.V., Gorbunov V.V. The Relationship of Endothelial Dysfunction with the Development of Diastolic Heart Failure in Patients with Hypertension. Rational Pharmacotherapy in Cardiology. 2020;16(3):370-376. (In Russ.) https://doi.org/10.20996/1819-6446-2020-05-04

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ISSN 1819-6446 (Print)
ISSN 2225-3653 (Online)