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Association of rs556621 Polymorphism with Development of Stroke in Patients with Cardiovascular Pathology

https://doi.org/10.20996/1819-6446-2019-15-5-634-640

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Abstract

Aim. To study the association of single nucleotide polymorphism rs556621 (G> T) with development of stroke in patients of the East Siberian population with cardiovascular pathology and risk factors.

Material and methods. The study involved 260 patients (157 men and 103 women) with stroke (mean age 57.0 [51.0-62.0]) and 272 patients (170 men and 102 women) of the control group (mean age 55.0 [51.0-62.0]). The examination of the main group included: collection of complaints, anamnesis, clinical examination, computed tomography of the brain, electrocardiography, echocardioscopy, ultrasound duplex scanning of extracranial brachiocephalic arteries, daily blood pressure and heart rate monitoring, analysis of the blood coagulation system. The patients of the main group have arterial hypertension, paroxysmal supraventricular tachycardias, dyslipidemia, atherosclerosis of the brachiocephalic arteries, disorders of the hemostatic system. The control group was studied in the framework of the HAPIEE international project. Molecular genetic research was performed by real-time polymerase chain reaction.

Results. There were no statistically significant differences in the frequencies of genotypes and single nucleotide polymorphism rs556621 alleles (G>T) in the subgroup of patients with stroke and those in the control group. The frequency of the rare TT genotype among patients with stroke was 13.3%±4.16, among healthy individuals – 8.8±3.37% (p=0.1). Gender differences when comparing the frequencies of genotypes and alleles were also not detected (p>0.05). The frequencies of the TT genotype were approximately the same in the subgroup of patients with arterial hypertension (13.1%±4.22) and in the control group (7.4±5.25%; p>0.05). No significant differences were observed in the frequencies of the rare genotype of the studied polymorphism in the subgroup of patients with supraventricular tachycardias (20.0±14.37%), hypercoagulability (15.9±7.64%) and the control group (8.8±3.37%), p>0.05. A statistically significant relationship was found between the rare genotype TT of single nucleotide polymorphism rs556621 (G>T) and the development of stroke in patients with dyslipidemia and atherosclerotic lesions of the coronary arteries (p=0.041; odds ratio 1.86, 95% confidence interval 1.02-3.41).

Conclusion. The genotype of TTs of single nucleotide polymorphism rs556621 (G> T) increases the risk of developing stroke in patients with dyslipidemia and atherosclerosis of the brachiocephalic arteries compared with carriers of the GG and GT genotypes. The obtained data are recommended to be considered when prescribing lipid-lowering and antithrombotic therapy. 

About the Authors

S. Yu. Nikulina
Krasnoyarsk State Medical University named after Professor V.F. Voyno-Yasenetsky
Russian Federation

MD, PhD, Professor, Acting Rector, Vice-Rector for Academic Affairs, Head of Chair of Internal Diseases №1,

Partizana Zheleznyaka ul. 1, Krasnoyarsk, 660022



V. A. Shulman
Krasnoyarsk State Medical University named after Professor V.F. Voyno-Yasenetsky
Russian Federation

MD, PhD, Professor, Chair of Internal Diseases №1, 

Partizana Zheleznyaka ul. 1, Krasnoyarsk, 660022



A. A. Chernova
Krasnoyarsk State Medical University named after Professor V.F. Voyno-Yasenetsky; Krasnoyarsk Interdistrict Clinical Hospital №20 named after I.S. Berzon
Russian Federation

MD, PhD, Associate Professor, Chair of Internal Diseases №1; Senior Researcher, Russian-Italian Laboratory of Medical Genetics, Research Institute of Molecular Medicine and Pathobiochemistry, Partizana Zheleznyaka ul. 1, Krasnoyarsk, 660022

Functional Diagnostics Physician



S. V. Prokopenko
Krasnoyarsk State Medical University named after Professor V.F. Voyno-Yasenetsky
Russian Federation

MD, PhD, Professor, Head of Chair of Nervous Diseases with a Course of Medical Rehabilitation, 

Partizana Zheleznyaka ul. 1, Krasnoyarsk, 660022



D. A. Nikulin
Krasnoyarsk State Medical University named after Professor V.F. Voyno-Yasenetsky; Federal Siberian Research Clinical Center, Federal Medical and Biological Agency of Russia
Russian Federation

MD, PhD, Assistant, Chair of Nervous Diseases with a Course of Medical Rehabilitation, Partizana Zheleznyaka ul. 1, Krasnoyarsk, 660022;

General Practitioner, Kolomenskaya ul. 26, Krasnoyarsk, 660037



I. M. Platunova
Krasnoyarsk Interdistrict Clinical Hospital №20 n.a. I.S. Berzon
Russian Federation

MD, PhD, Neurologist,

Instrumentalnaya ul. 12, Krasnoyarsk, 660123



S. S. Tretyakova
Krasnoyarsk State Medical University named after Professor V.F. Voyno-Yasenetsky
Russian Federation

MD, PhD, Laboratory Assistant, Russian-Italian Laboratory of Medical Genetics, Scientific Research Institute of Molecular Medicine and Pathobiochemistry, 

Partizana Zheleznyaka ul. 1, Krasnoyarsk, 660022



V. N. Maksimov
Institute of Internal and Preventive Medicine, Federal Research Center Institute Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences
Russian Federation

MD, PhD, Professor, Head of Laboratory of Molecular Genetic Studies of Therapeutic Diseases,

Borisa Bogatkova ul. 175-1, Novosibirsk, 630089 



A. A. Gurazheva
Institute of Internal and Preventive Medicine, Federal Research Center Institute Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences
Russian Federation

Junior Researcher, Laboratory of Molecular Genetic Studies of Therapeutic Diseases,

Borisa Bogatkova ul. 175-1, Novosibirsk, 630089 



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For citation:


Nikulina S.Y., Shulman V.A., Chernova A.A., Prokopenko S.V., Nikulin D.A., Platunova I.M., Tretyakova S.S., Maksimov V.N., Gurazheva A.A. Association of rs556621 Polymorphism with Development of Stroke in Patients with Cardiovascular Pathology. Rational Pharmacotherapy in Cardiology. 2019;15(5):634-640. (In Russ.) https://doi.org/10.20996/1819-6446-2019-15-5-634-640

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ISSN 1819-6446 (Print)
ISSN 2225-3653 (Online)