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Aim. To study the effects of furostanol glycosides from cultured Dioscorea Deltoidea cells (DM-05, Institute of Plant Physiology, RAS) on physiological and biochemical markers of endothelial function in rats with hypoestrogen-induced endothelial dysfunction.

Material and methods. 10 female rats of Wistar line, with body mass 200-300 g have been included in the experiment. The bilateral ovariectomy was performed in rats to produce the model of hypoestrogen-induced endothelial dysfunction. Rats were treated with the injections of DM-05 during 6 weeks. False ovariectomy was performed in rats of control group (n=10).

Results. DM-05 restored the levels of stable metabolites of nitric oxide (NO) which reflex endothelial NO-synthase activity. Besides DM-05 corrected blood pressure and endothelial function. Experiments on open heart showed that DM-05 protects the cardiac tissue from hypoestrogen-induced hyperadrenoreactivity.

Conclusion. Treatment with plant origin substances with estrogen-like activity can be a perspective approach to the correction of endothelial function and decrease in cardiovascular risk in menopause women.

About the Authors

E. B. Artyushkova
Kursk State Medical University, Kursk
Russian Federation

N. G. Gumanova
State Research center of preventive medicine of Rosmedtechnology, Moscow
Russian Federation

V. A. Metelskaya
State Research center of preventive medicine of Rosmedtechnology, Moscow
Russian Federation

T. G. Pokrovskaya
Kursk State Medical University, Kursk
Russian Federation

V. I. Kochkarov
Kursk State Medical University, Kursk
Russian Federation

M. V. Korokin
Kursk State Medical University, Kursk
Russian Federation

M. V. Pokrovsky
Kursk State Medical University, Kursk
Russian Federation

L. V. Korokina
Kursk State Medical University, Kursk
Russian Federation

A. M. Nosov
Institute of Plant Physiology, Russian Academy of Sciences, Moscow
Russian Federation

M. M. Korneev
Kursk State Medical University, Kursk
Russian Federation


1. Miller AM, Wilbur J, Chandler PJ, Sorokin O. Cardiovascular disease risk factors and menopausal status in midlife women from the former Soviet Union. Women Health 2003;38:19-36.

2. Sjöberg L, Kaaja R, Tuomilehto J. Epidemiology of postmenopausal hypertension. Int J Clin Pract Suppl 2004;139:4-12.

3. Creatsas G, Christodoulakos G, Lambrinoudaki I. Cardiovascular disease: screening and management of the a-symptomatic high-risk post-menopausal woman. Maturitas 2005; 52 Suppl 1: S32-S37.

4. Lobo RA. Surgical menopause and cardiovascular risks. Menopause 2007;14(3 Pt 2):562-

5. Rosano GM, Vitale C, Marazzi G, Volterrani M. Menopause and cardiovascular disease: the evidence. Climacteric 2007;10 Suppl 1:19-24.

6. Burt V.L. Whelton P., Roccella E. J., et al. Prevalence of hypertension in the US adult population. Results from the Third National Health and Nutrition Examination Survey 1991. Hypertension 1995; 25(3): 303–4.

7. Pradhan AD, Manson JE, Rossouw JE, et al. Inflammatory biomarkers, hormone replacement therapy, and incident coronary heart disease: prospective analysis from the Women's Health Initiative observational study. JAMA 2002; 288:980-7.

8. Shakir Y.A., Samsioe G., Nyberg P., et al. Do sex hormones influence features of the metabolic syndrome in middle-aged women? A population-based study of Swedish women: the Women's Health in the Lund Area (WHILA) Study. Fertil Steril 2007; 88(1):163-71.

9. LaRosa JC. Lipids and cardiovascular disease: do the findings and therapy apply equally to men and women? Womens Health Issues 1992; 2:102-11.

10. Вихляева Е.М. Климактерический синдром. В: Жмакина К. Н., редактор. Гинекологическая эндокринология. М.: Медицина; 1989.

11. Барт Б. Я., Бороненков, В. Ф. Беневская Г. М. Артериальная гипертония у женщин в постменопаузе: современные возможности медикаментозной терапии в поликлинических условиях. Росс кардиол журн 2001; (5): 69–70.

12. Zárate A, Basurto L, Hernández M. The metabolic syndrome in postmenopausal women. Clinical implications [in Spanish]. Gac Med Mex 2003;139:625-8.

13. Быстрова М.М., Бритов А.Н., Горбунов В.М. и др. Заместительная гормо- нальная терапия у женщин с артериальной гипертонией в пери- и постме- нопаузе: гемодинамические эффекты. Тер арх 2001;73(10): 33–8.

14. Davison S., Davis S.R. New markers for cardiovascular disease risk in women: impact of endogenous estrogen status and exogenous postmenopausal hormone therapy. J Clin Endocrinol Metab 2003; 88:2470-8.

15. Sowers M.R., Matthews K.A., Jannausch M., et al. Hemostatic factors and estrogen during the menopausal transition. J Clin Endocrinol Metab 2005;90:5942-8.

16. Reimann M., Vafeiadou K., Hall W.L., et al. Evidence for associations between common polymorphisms of estrogen receptor beta gene with homocysteine and nitric oxide. Climacteric 2006; 9:215-223.

17. Горовиц М.Б., Абубакиров Н.К., Волернер Ю.С. Химия спиростанолов. М.: Наука; 1996.

18. Sirtori C.R. Risks and benefits of soy phytoestrogens in cardiovascular diseases, cancer, climacteric symptoms and osteoporosis. Drug Saf 2001; 24:665-82.

19. Cassidy A., Albertazzi P., Lise Nielsen I., et al. Critical review of health effects of soyabean phyto-oestrogens in post-menopausal women. Proc Nutr Soc 2006; 65:76-92.

20. Vico L. Dolleans E., Benhamou C.L., Courteix D. Dose effects of propranolol on cancellous and cortical bone in ovariectomized adult rats. J Pharmacol Exp 2005; 10:110-1.

21. Laursen J. B., Rajagopalan S.,Galis Z., et al. Role of superoxide in angiotensin IIinduced but not catecholamine-induced hypertension. Circulation 1997; 95(3):588-93.

22. Галаган М.Е., Широколова А. В., Ванин А.Ф. Гипотензивное действие оксида азота, продуцируемого из экзо-и эндогенных источников. Вопр мед химии 1991; 37(1): 67-70.

23. Покровский М. В., Качкаров В. И., Покровская Т. Г. и др. Методические подходы для количественной оценки развития эндотелиальной дисфункции при L-NAME- индуцированной модели дефицита оксида азота в эксперименте. Кубанский научно-медицинский вестник 2006;(12): 25-9.

24. Гуманова Н.Г., Артюшкова Е.Б., Метельская В.А., и др. Влияние антиоксидантов Q510 и резвератрола на регуляторную функцию эндотелия у крыс с моделированной артериальной гипертонией. Бюлл эксп биол мед 2007; 143 (6): 619-22.

25. Метельская В. А., Гуманова Н. Г. Скрининг-метод определения уровня метаболитов оксида азота в сыворотке крови человека. Клин лабор диагн 2005;(6):15-18.

26. Granger D.L., Anstey N.M., Miller W.C., et al. Measuring nitric oxide production in human clinical studies. Methods Enzymol 1999;301: 49-61.

27. Fleming I., Busse R. NO: the primary EDRF. J Mol Cell Cardiol 1999; 31(1): 5- 14.

28. Oldenhave A., Jaszmann L.J., Haspels A.A., et al. Impact of climacteric on wellbeing, a survey based on 5213 women from 39 to 60 years old. Am J Obstet Gynecol 1993; 168(3 Pt 1): 772-80.

29. van der Schouw Y.T., de Kleijn M..J, Peeters P.H., Grobbee D.E. Phyto-oestrogens and cardiovascular disease risk. Nutr Metab Cardiovasc Dis 2000;10:154-67.

30. Lange Y, Ye J, Steck TL. Activation of membrane cholesterol by displacement from phospholipids. J Biol Chem. 2005; 280: 36126-31.

31. Petit PR, Sauvaire YD, Hillaire-Buys DM, et al Steroid saponins from fenugreek seeds: extraction, purification, and pharmacological investigation on feeding behavior and plasma cholesterol. Steroids. 1995;60:674-80.

For citation:

Artyushkova E.B., Gumanova N.G., Metelskaya V.A., Pokrovskaya T.G., Kochkarov V.I., Korokin M.V., Pokrovsky M.V., Korokina L.V., Nosov A.M., Korneev M.M. EFFECT OF FUROSTANOL GLYCOSIDES FROM CULTURED DIOSCOREA DELTOIDEA CELLS ON REGULATORY FUNCTION OF ENDOTHELIUM IN A RAT MODEL OF HYPOESTROGEN-INDUCED ENDOTHELIAL DYSFUNCTION. Rational Pharmacotherapy in Cardiology. 2008;4(2):85-88. (In Russ.)

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