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EFFECT OF FUROSTANOL GLYCOSIDES FROM CULTURED DIOSCOREA DELTOIDEA CELLS ON REGULATORY FUNCTION OF ENDOTHELIUM IN A RAT MODEL OF HYPOESTROGEN-INDUCED ENDOTHELIAL DYSFUNCTION

https://doi.org/10.20996/1819-6446-2008-4-2-85-88

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Abstract

Aim. To study the effects of furostanol glycosides from cultured Dioscorea Deltoidea cells (DM-05, Institute of Plant Physiology, RAS) on physiological and biochemical markers of endothelial function in rats with hypoestrogen-induced endothelial dysfunction.

Material and methods. 10 female rats of Wistar line, with body mass 200-300 g have been included in the experiment. The bilateral ovariectomy was performed in rats to produce the model of hypoestrogen-induced endothelial dysfunction. Rats were treated with the injections of DM-05 during 6 weeks. False ovariectomy was performed in rats of control group (n=10).

Results. DM-05 restored the levels of stable metabolites of nitric oxide (NO) which reflex endothelial NO-synthase activity. Besides DM-05 corrected blood pressure and endothelial function. Experiments on open heart showed that DM-05 protects the cardiac tissue from hypoestrogen-induced hyperadrenoreactivity.

Conclusion. Treatment with plant origin substances with estrogen-like activity can be a perspective approach to the correction of endothelial function and decrease in cardiovascular risk in menopause women.

About the Authors

E. B. Artyushkova
Kursk State Medical University, Kursk
Russian Federation


N. G. Gumanova
State Research center of preventive medicine of Rosmedtechnology, Moscow
Russian Federation


V. A. Metelskaya
State Research center of preventive medicine of Rosmedtechnology, Moscow
Russian Federation


T. G. Pokrovskaya
Kursk State Medical University, Kursk
Russian Federation


V. I. Kochkarov
Kursk State Medical University, Kursk
Russian Federation


M. V. Korokin
Kursk State Medical University, Kursk
Russian Federation


M. V. Pokrovsky
Kursk State Medical University, Kursk
Russian Federation


L. V. Korokina
Kursk State Medical University, Kursk
Russian Federation


A. M. Nosov
Institute of Plant Physiology, Russian Academy of Sciences, Moscow
Russian Federation


M. M. Korneev
Kursk State Medical University, Kursk
Russian Federation


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For citation:


Artyushkova E.B., Gumanova N.G., Metelskaya V.A., Pokrovskaya T.G., Kochkarov V.I., Korokin M.V., Pokrovsky M.V., Korokina L.V., Nosov A.M., Korneev M.M. EFFECT OF FUROSTANOL GLYCOSIDES FROM CULTURED DIOSCOREA DELTOIDEA CELLS ON REGULATORY FUNCTION OF ENDOTHELIUM IN A RAT MODEL OF HYPOESTROGEN-INDUCED ENDOTHELIAL DYSFUNCTION. Rational Pharmacotherapy in Cardiology. 2008;4(2):85-88. (In Russ.) https://doi.org/10.20996/1819-6446-2008-4-2-85-88

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